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Selection of Reliable Reference Genes for Gene Expression Studies on Rhododendron molle G. Don

Overview of attention for article published in Frontiers in Plant Science, October 2016
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Title
Selection of Reliable Reference Genes for Gene Expression Studies on Rhododendron molle G. Don
Published in
Frontiers in Plant Science, October 2016
DOI 10.3389/fpls.2016.01547
Pubmed ID
Authors

Zheng Xiao, Xiaobo Sun, Xiaoqing Liu, Chang Li, Lisi He, Shangping Chen, Jiale Su

Abstract

The quantitative real-time polymerase chain reaction (qRT-PCR) approach has become a widely used method to analyze expression patterns of target genes. The selection of an optimal reference gene is a prerequisite for the accurate normalization of gene expression in qRT-PCR. The present study constitutes the first systematic evaluation of potential reference genes in Rhododendron molle G. Don. Eleven candidate reference genes in different tissues and flowers at different developmental stages of R. molle were assessed using the following three software packages: GeNorm, NormFinder, and BestKeeper. The results showed that EF1-α (elongation factor 1-alpha), 18S (18s ribosomal RNA), and RPL3 (ribosomal protein L3) were the most stable reference genes in developing rhododendron flowers and, thus, in all of the tested samples, while tublin (TUB) was the least stable. ACT5 (actin), RPL3, 18S, and EF1-α were found to be the top four choices for different tissues, whereas TUB was not found to favor qRT-PCR normalization in these tissues. Three stable reference genes are recommended for the normalization of qRT-PCR data in R. molle. Furthermore, the expression profiles of RmPSY (phytoene synthase) and RmPDS (phytoene dehydrogenase) were assessed using EF1-α, 18S, ACT5, RPL3, and their combination as internals. Similar trends were found, but these trends varied when the least stable reference gene TUB was used. The results further prove that it is necessary to validate the stability of reference genes prior to their use for normalization under different experimental conditions. This study provides useful information for reliable qRT-PCR data normalization in gene studies of R. molle.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 26%
Student > Doctoral Student 2 11%
Student > Master 2 11%
Researcher 2 11%
Professor 1 5%
Other 2 11%
Unknown 5 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 42%
Biochemistry, Genetics and Molecular Biology 4 21%
Medicine and Dentistry 1 5%
Unknown 6 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2016.
All research outputs
#20,346,264
of 22,893,031 outputs
Outputs from Frontiers in Plant Science
#16,199
of 20,304 outputs
Outputs of similar age
#273,551
of 316,298 outputs
Outputs of similar age from Frontiers in Plant Science
#275
of 390 outputs
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