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Development of Novel SSR Markers for Flax (Linum usitatissimum L.) Using Reduced-Representation Genome Sequencing

Overview of attention for article published in Frontiers in Plant Science, January 2017
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Title
Development of Novel SSR Markers for Flax (Linum usitatissimum L.) Using Reduced-Representation Genome Sequencing
Published in
Frontiers in Plant Science, January 2017
DOI 10.3389/fpls.2016.02018
Pubmed ID
Authors

Jianzhong Wu, Qian Zhao, Guangwen Wu, Shuquan Zhang, Tingbo Jiang

Abstract

Flax (Linum usitatissimum L.) is a major fiber and oil yielding crop grown in northeastern China. Identification of flax molecular markers is a key step toward improving flax yield and quality via marker-assisted breeding. Simple sequence repeat (SSR) markers, which are based on genomic structural variation, are considered the most valuable type of genetic marker for this purpose. In this study, we screened 1574 microsatellites from Linum usitatissimum L. obtained using reduced representation genome sequencing (RRGS) to systematically identify SSR markers. The resulting set of microsatellites consisted mainly of trinucleotide (56.10%) and dinucleotide (35.23%) repeats, with each motif consisting of 5-8 repeats. We then evaluated marker sensitivity and specificity based on samples of 48 flax isolates obtained from northeastern China. Using the new SSR panel, the results demonstrated that fiber flax and oilseed flax varieties clustered into two well separated groups. The novel SSR markers developed in this study show potential value for selection of varieties for use in flax breeding programs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 5%
France 1 5%
Unknown 18 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 25%
Student > Master 3 15%
Researcher 2 10%
Student > Bachelor 1 5%
Unspecified 1 5%
Other 2 10%
Unknown 6 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 50%
Unspecified 1 5%
Arts and Humanities 1 5%
Biochemistry, Genetics and Molecular Biology 1 5%
Sports and Recreations 1 5%
Other 0 0%
Unknown 6 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2017.
All research outputs
#20,403,545
of 22,953,506 outputs
Outputs from Frontiers in Plant Science
#16,278
of 20,383 outputs
Outputs of similar age
#356,914
of 421,774 outputs
Outputs of similar age from Frontiers in Plant Science
#397
of 529 outputs
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We're also able to compare this research output to 529 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.