Natalia Moroz, Karen R. Fritch, Matthew J. Marcec, Diwaker Tripathi, Andrei Smertenko, Kiwamu Tanaka
Abstract
A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists (Phytophthora infestans and Spongospora subterranea) and fungi (Verticillium dahliae and Colletotrichum coccodes). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes.
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 February 2017.
All research outputs
#6,304,022
of 23,776,941 outputs
Outputs from Frontiers in Plant Science
#3,313
of 21,845 outputs
Outputs of similar age
#115,334
of 422,632 outputs
Outputs of similar age from Frontiers in Plant Science
#82
of 510 outputs
Altmetric has tracked 23,776,941 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 21,845 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 84% of its peers.
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We're also able to compare this research output to 510 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.
