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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
|
|---|---|
| Published in |
Frontiers in Plant Science, March 2018
|
| DOI | 10.3389/fpls.2018.00371 |
| Pubmed ID | |
| Authors |
Dominik Novák, Pavol Vadovič, Miroslav Ovečka, Olga Šamajová, George Komis, Jean Colcombet, Jozef Šamaj |
| Abstract |
Phospholipase D alpha 1 (PLDα1, At3g15730) and its product phosphatidic acid (PA) are involved in a variety of cellular and physiological processes, such as cytoskeletal remodeling, regulation of stomatal closure and opening, as well as biotic and abiotic stress signaling. Here we aimed to study developmental expression patterns and subcellular localization of PLDα1 in Arabidopsis using advanced microscopy methods such as light-sheet fluorescence microscopy (LSFM) and structured illumination microscopy (SIM). We complemented two knockoutpldα1mutants with a YFP-tagged PLDα1 expressed under thePLDα1native promoter in order to study developmental expression pattern and subcellular localization of PLDα1 inArabidopsis thalianaunder natural conditions. Imaging of tissue-specific and developmentally-regulated localization of YFP-tagged PLDα1 by LSFM in roots of growing seedlings showed accumulation of PLDα1-YFP in the root cap and the rhizodermis. Expression of PLDα1-YFP in the rhizodermis was considerably higher in trichoblasts before and during root hair formation and growth. Thus, PLDα1-YFP accumulated in emerging root hairs and in the tips of growing root hairs. PLDα1-YFP showed cytoplasmic subcellular localization in root cap cells and in cells of the root transition zone. In aerial parts of plants PLDα1-YFP was also localized in the cytoplasm showing enhanced accumulation in the cortical cytoplasmic layer of epidermal non-dividing cells of hypocotyls, leaves, and leaf petioles. However, in dividing cells of root apical meristem and leaf petiole epidermis PLDα1-YFP was enriched in mitotic spindles and phragmoplasts, as revealed by co-visualization with microtubules. Finally, super-resolution SIM imaging revealed association of PLDα1-YFP with both microtubules and clathrin-coated vesicles (CCVs) and pits (CCPs). In conclusion, this study shows the developmentally-controlled expression and subcellular localization of PLDα1 in dividing and non-dividing Arabidopsis cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 9% |
| Germany | 3 | 5% |
| United Kingdom | 3 | 5% |
| Netherlands | 3 | 5% |
| China | 2 | 4% |
| Australia | 2 | 4% |
| Ireland | 1 | 2% |
| Czechia | 1 | 2% |
| France | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 32 | 58% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 26 | 47% |
| Scientists | 24 | 44% |
| Science communicators (journalists, bloggers, editors) | 5 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 29% |
| Student > Ph. D. Student | 9 | 26% |
| Student > Doctoral Student | 3 | 9% |
| Other | 2 | 6% |
| Student > Bachelor | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 6 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 35% |
| Agricultural and Biological Sciences | 11 | 32% |
| Medicine and Dentistry | 2 | 6% |
| Environmental Science | 1 | 3% |
| Neuroscience | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 7 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 33. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2019.
All research outputs
#1,294,369
of 26,588,416 outputs
Outputs from Frontiers in Plant Science
#368
of 25,353 outputs
Outputs of similar age
#27,434
of 351,800 outputs
Outputs of similar age from Frontiers in Plant Science
#10
of 471 outputs
Altmetric has tracked 26,588,416 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 25,353 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,800 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 471 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.