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X Demographics
Mendeley readers
Attention Score in Context
| Title |
An Efficient Protocol for Model Legume Root Protoplast Isolation and Transformation
|
|---|---|
| Published in |
Frontiers in Plant Science, June 2018
|
| DOI | 10.3389/fpls.2018.00670 |
| Pubmed ID | |
| Authors |
Ning Jia, Yali Zhu, Fang Xie |
| Abstract |
Transient gene expression systems using protoplasts have been widely used for rapid functional characterization of genes and high-throughput analysis in many model and crop species. Here, we describe a simplified and highly efficient root protoplast isolation and transient expression system in the model legumes Lotus japonicus and Medicago truncatula. Firstly, we presented an efficient protocol for isolating protoplasts from L. japonicus and M. truncatula roots. We then established an efficient transient expression system in these legumes root protoplasts. Using this protocol, the subcellular localization of two symbiosis related proteins (SYMRK and ERN1) were visualized in the plasma membrane and nuclei, respectively. Collectively, this efficient protoplast isolation and transformation protocol is sufficient for studies on protein subcellular localization, and should be suitable for many other molecular biology applications. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 20% |
| Canada | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 89 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 17% |
| Student > Ph. D. Student | 14 | 16% |
| Student > Bachelor | 12 | 13% |
| Student > Master | 10 | 11% |
| Other | 3 | 3% |
| Other | 11 | 12% |
| Unknown | 24 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 35 | 39% |
| Biochemistry, Genetics and Molecular Biology | 23 | 26% |
| Unspecified | 2 | 2% |
| Environmental Science | 1 | 1% |
| Psychology | 1 | 1% |
| Other | 2 | 2% |
| Unknown | 25 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2018.
All research outputs
#14,417,376
of 23,090,520 outputs
Outputs from Frontiers in Plant Science
#8,292
of 20,702 outputs
Outputs of similar age
#186,723
of 329,882 outputs
Outputs of similar age from Frontiers in Plant Science
#223
of 476 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,702 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,882 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 476 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.