Matthew L. Broadhead, Zerina Lokmic, Mei Lin Tan, Andrew Stevenson, David S. Binns, Carleen Cullinane, Rodney J. Hicks, Peter F. M. Choong, Damian E. Myers
Abstract
Reliable animal models are required to evaluate novel treatments for osteosarcoma. In this study, the aim was to implement advanced imaging techniques in a murine model of orthotopic osteosarcoma to improve disease modeling and the assessment of primary and metastatic disease.
Intra-tibial injection of luciferase-tagged OPGR80 murine osteosarcoma cells was performed in Balb/c nude mice. Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity. Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET.
[(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease. Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively. In vivo bioluminescent assay showed that systemically delivered PEDF inhibited growth of primary osteosarcoma.
Application of [(18)F]-Fluoride-PET and [(18)F]-FDG-PET to an established murine model of orthotopic osteosarcoma has improved the assessment of disease. The use of targeted imaging should prove beneficial for the evaluation of new approaches to osteosarcoma therapy.
Pharmacology, Toxicology and Pharmaceutical Science
1
7%
Veterinary Science and Veterinary Medicine
1
7%
Agricultural and Biological Sciences
1
7%
Other
3
20%
Unknown
2
13%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 August 2015.
All research outputs
#18,420,033
of 22,818,766 outputs
Outputs from Frontiers in Surgery
#918
of 2,858 outputs
Outputs of similar age
#189,802
of 263,982 outputs
Outputs of similar age from Frontiers in Surgery
#9
of 21 outputs
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