Title |
Effects of dietary valine:lysine ratio on the performance, amino acid composition of tissues and mRNA expression of genes involved in branched-chain amino acid metabolism of weaned piglets
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Published in |
Animal Bioscience, May 2017
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DOI | 10.5713/ajas.17.0148 |
Pubmed ID | |
Authors |
Ye Tong Xu, Xiao Kang, Chun Lin Wang, Ming Feng Yuan, Xiang Shu Piao |
Abstract |
The goal of this study was to investigate the effects of dietary standard ileal digestible (SID) valine:lysine ratios on performance, intestinal morphology, amino acids of liver and muscle, plasma indices and mRNA expression of branched-chain amino acid (BCAA) metabolism enzymes. A total of 144 crossbred pigs (Duroc × Landrace × Large White) weaned at 28 ± 4 days of age (8.79 ± 0.02 kg body weight) were randomly allotted to 1 of 4 diets formulated to provide SID valine: lysine ratios of 50, 60, 70 or 80%. Each diet was fed to 6 pens of pigs with 6 pigs per pen (3 gilts and 3 barrows) for 28 days. Average daily gain increased quadratically (P < 0.05), the villous height of the duodenum, jejunum and ileum increased linearly (P < 0.05) as the SID valine: lysine ratio increased. The concentrations of plasma α-keto isovaleric and valine increased linearly (P < 0.05), plasma aspartate, asparagine, cysteine and glutamine decreased (P < 0.05) as the SID valine: lysine ratio increased. An increase in SID lysine: valine levels increased mRNA expression levels of mitochondrial BCAA transaminase and branched-chain α-keto acid dehydrogenase in the longissimus dorsi muscle (P < 0.05). Using a quadratic model, a SID valine: lysine ratio of 68% was shown to maximize the growth of weaned pigs which is slightly higher than the level recommended by the National Research Council (2012). |
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