Chapter title |
From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.
|
---|---|
Chapter number | 12 |
Book title |
Structural Proteomics
|
Published in |
Methods in molecular biology, January 2021
|
DOI | 10.1007/978-1-0716-1406-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-07-161405-1, 978-1-07-161406-8
|
Authors |
Diebolder, Christoph A, Dillard, Rebecca S, Renault, Ludovic, Christoph A. Diebolder, Rebecca S. Dillard, Ludovic Renault, Diebolder, Christoph A., Dillard, Rebecca S. |
Abstract |
In this chapter, we present an overview of a standard protocol to achieve structure determination at high resolution by Single Particle Analysis cryogenic Electron Microscopy using apoferritin as a standard sample. The purified apoferritin is applied to a glow-discharged support and then flash frozen in liquid ethane. The prepared grids are loaded into the electron microscope and checked for particle spreading and ice thickness. The microscope alignments are performed and the data collection session is setup for an overnight data collection. The collected movies containing two-dimensional images of the apoferritin sample are then processed to obtain a high-resolution three-dimensional reconstruction. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 33% |
Student > Ph. D. Student | 1 | 17% |
Other | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Arts and Humanities | 1 | 17% |
Immunology and Microbiology | 1 | 17% |
Unknown | 2 | 33% |