Chapter title |
The Nucleolus
|
---|---|
Chapter number | 2 |
Book title |
The Nucleolus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3792-9_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3790-5, 978-1-4939-3792-9
|
Authors |
Lanctôt, Christian, Christian Lanctôt Ph.D., Christian Lanctôt |
Editors |
Attila Németh |
Abstract |
Caenorhabditis elegans is a well-established model organism which allows, among others, to investigate the link between nucleolar structure/function on the one hand and cell fate choices and cellular differentiation on the other. In addition, C. elegans can be used to study the role of the nucleolus in processes that can be difficult to faithfully reproduce in vitro, such as gametogenesis, disease development, and aging. Here I present two complementary techniques, immunofluorescent staining and DNA fluorescence in situ hybridization, that have been adapted to label nucleolar components at various stages of the life cycle of the worm. |
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