We are using mESCs to study long 3'UTR function in neurons. Here is a simple method from @bae_bbm to generate long 3'UTR deletion mutants in mESCs using CRISPR. mES-neurons express long 3'UTRs at abundance levels found in brain tissues. https://t.co/UVYMAw
881 followers
168 followers
The authors of "CRISPR-Mediated Knockout of Long 3′ UTR mRNA Isofo…" (https://t.co/FaM6T42Asx) included RRIDs in their paper! RRIDs like this improve reproducibility in scientific research. #ReproducibleResearch #accelerateopenscience
335 followers
nanopore: CRISPR-Mediated Knockout of Long 3' UTR mRNA Isoforms in mESC-Derived Neurons https://t.co/9WexxomACw
1,086 followers
CRISPR-Mediated Knockout of Long 3' UTR mRNA Isoforms in mESC-Derived Neurons https://t.co/DbMF391sWE https://t.co/RyJzdnmApf