Title |
Immunogenicity and Protective Response Induced by Recombinant Plasmids Based on the BAB1_0267 and BAB1_0270 Open Reading Frames of Brucella abortus 2308 in BALB/c Mice
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, September 2016
|
DOI | 10.3389/fcimb.2016.00117 |
Pubmed ID | |
Authors |
Leonardo A. Gómez, Francisco I. Alvarez, Pablo A. Fernández, Manuel R. Flores, Raúl E. Molina, Roberto F. Coloma, Angel A. Oñate |
Abstract |
Immunogenicity induced by recombinant plasmids based on the BAB1_0267 and BAB1_0270 open reading frames (ORFs) of Brucella abortus 2308 was evaluated. Bioinformatics analyses indicate that the BAB1_0267 and BAB1_0270 ORFs encode a protein with a SH3 domain and a Zn-dependent metalloproteinase, respectively. Both ORFs have important effects on intracellular survival and replication of B. abortus 2308, mediated via professional and non-professional phagocytic cells. Our results show that immunization with the recombinant plasmid based on the BAB1_0267 ORF significantly increases the production of IgG1, levels of IFN-γ and the lymphoproliferative response of splenocytes. However, BAB1_0267 did not provide significant levels of protection. The plasmid based on the BAB1_0270 significantly increased IgG2a production, levels of IFN-γ and TNF-α, and the lymphoproliferative response of splenocytes. These results demonstrate that immunization with the BAB1_0270 derived recombinant plasmid induce a Th1-type immune response, correlated with a heightened resistance to B. abortus 2308 infection in mice. It is concluded that the Th1-type immune response against bacterial Zn-dependent metalloproteinase induces a protective response in mice, and that pV270 recombinant plasmid is an effective candidate microbicide against brucellosis. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 31% |
Student > Master | 3 | 19% |
Researcher | 3 | 19% |
Student > Bachelor | 1 | 6% |
Student > Postgraduate | 1 | 6% |
Other | 0 | 0% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 4 | 25% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Veterinary Science and Veterinary Medicine | 2 | 13% |
Linguistics | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Other | 3 | 19% |
Unknown | 3 | 19% |