Title |
An Environmental DNA Primer for Microbial and Restoration Ecology
|
---|---|
Published in |
Microbial Ecology, February 2023
|
DOI | 10.1007/s00248-022-02168-5 |
Pubmed ID | |
Authors |
Michael Tessler, Seth W. Cunningham, Melissa R. Ingala, Sally D. Warring, Mercer R. Brugler |
Abstract |
Environmental DNA (eDNA) sequencing-DNA collected from the environment from living cells or shed DNA-was first developed for working with microbes and has greatly benefitted microbial ecologists for decades since. These tools have only become increasingly powerful with the advent of metabarcoding and metagenomics. Most new studies that examine diverse assemblages of bacteria, archaea, protists, fungi, and viruses lean heavily into eDNA using these newer technologies, as the necessary sequencing technology and bioinformatic tools have become increasingly affordable and user friendly. However, eDNA methods are rapidly evolving, and sometimes it can feel overwhelming to simply keep up with the basics. In this review, we provide a starting point for microbial ecologists who are new to DNA-based methods by detailing the eDNA methods that are most pertinent, including study design, sample collection and storage, selecting the right sequencing technology, lab protocols, equipment, and a few bioinformatic tools. Furthermore, we focus on how eDNA work can benefit restoration and what modifications are needed when working in this subfield. |
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Geographical breakdown
Country | Count | As % |
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United States | 4 | 15% |
United Kingdom | 1 | 4% |
Unknown | 21 | 81% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 16 | 62% |
Scientists | 10 | 38% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 17% |
Unspecified | 4 | 13% |
Student > Doctoral Student | 3 | 10% |
Student > Master | 3 | 10% |
Student > Bachelor | 1 | 3% |
Other | 2 | 7% |
Unknown | 12 | 40% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 23% |
Unspecified | 4 | 13% |
Environmental Science | 3 | 10% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Chemistry | 1 | 3% |
Other | 1 | 3% |
Unknown | 12 | 40% |