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Directed Evolution

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Cover of 'Directed Evolution'

Table of Contents

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    Book Overview
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    Chapter 1 Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach
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    Chapter 2 Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR)
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    Chapter 3 Nondegenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization
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    Chapter 4 Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries
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    Chapter 5 SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution
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    Chapter 6 Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis
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    Chapter 7 GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes
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    Chapter 8 Rapid Cloning of Random Mutagenesis Libraries Using PTO-QuickStep
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    Chapter 9 Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs
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    Chapter 10 Application of Restriction Free (RF) Cloning in Circular Permutation
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    Chapter 11 Site-Directed Mutagenesis Method Mediated by Cas9
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    Chapter 12 Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity
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    Chapter 13 A Screening Method for P450 BM3 Mutant Libraries Using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds.
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    Chapter 14 Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screening Method
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    Chapter 15 Learning Strategies in Protein Directed Evolution
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Title
Directed Evolution
Published by
Springer US, February 2022
DOI 10.1007/978-1-0716-2152-3
ISBNs
978-1-07-162151-6, 978-1-07-162152-3
Editors

Currin, Andrew, Swainston, Neil

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