Chapter title |
RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines.
|
---|---|
Chapter number | 5 |
Book title |
RNA Vaccines
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6481-9_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6479-6, 978-1-4939-6481-9
|
Authors |
Susanne Rauch, Johannes Lutz, Aleksandra Kowalczyk, Thomas Schlake, Regina Heidenreich, Rauch, Susanne, Lutz, Johannes, Kowalczyk, Aleksandra, Schlake, Thomas, Heidenreich, Regina |
Editors |
Thomas Kramps, Knut Elbers |
Abstract |
Developing effective mRNA vaccines poses certain challenges concerning mRNA stability and ability to induce sufficient immune stimulation and requires a specific panel of techniques for production and testing. Here, we describe the production of stabilized mRNA with enhanced immunogenicity, generated using conventional nucleotides only, by introducing changes to the mRNA sequence and by complexation with the nucleotide-binding peptide protamine (RNActive® technology). Methods described here include the synthesis, purification, and protamine complexation of mRNA vaccines as well as a comprehensive panel of in vitro and in vivo methods for evaluation of vaccine quality and immunogenicity. |
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