TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method

Overview of attention for article published in Frontiers in Bioengineering and Biotechnology, August 2023

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It works. New cloning protocol TLTC tested with students. Very easy and cheap. Only diluted T5 exonuclease for 5 min on ice.

@MathSRIsh Ça marche ! Grosse simplification du protocole de clonage en utilisant une T5 exonucléase à 4°C. La publi. https://t.co/3tuzgcuXvJ

RT @FrontBioeng: New Research: TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method #bioengineering #biotech https://t.co/don…

RT @paperperday: "inserts and linear vectors was mixed at a molar ratio of approximately 3:1 and treated with 0.5 U of T5 exonuclease at 0°…

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