Title |
3D Gel Map of Arabidopsis Complex I
|
---|---|
Published in |
Frontiers in Plant Science, January 2013
|
DOI | 10.3389/fpls.2013.00153 |
Pubmed ID | |
Authors |
Katrin Peters, Katharina Belt, Hans-Peter Braun |
Abstract |
Complex I has a unique structure in plants and includes extra subunits. Here, we present a novel study to define its protein constituents. Mitochondria were isolated from Arabidopsis thaliana cell cultures, leaves, and roots. Subunits of complex I were resolved by 3D blue-native (BN)/SDS/SDS-PAGE and identified by mass spectrometry. Overall, 55 distinct proteins were found, seven of which occur in pairs of isoforms. We present evidence that Arabidopsis complex I consists of 49 distinct types of subunits, 40 of which represent homologs of bovine complex I. The nine other subunits represent special proteins absent in the animal linage of eukaryotes, most prominently a group of subunits related to bacterial gamma-type carbonic anhydrases. A GelMap http://www.gelmap.de/arabidopsis-3d-complex-i/ is presented for promoting future complex I research in Arabidopsis thaliana. |
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Unknown | 1 | 100% |
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Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 2% |
Unknown | 47 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 9 | 19% |
Student > Bachelor | 8 | 17% |
Student > Ph. D. Student | 8 | 17% |
Student > Master | 8 | 17% |
Student > Doctoral Student | 3 | 6% |
Other | 7 | 15% |
Unknown | 5 | 10% |
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Environmental Science | 1 | 2% |
Other | 2 | 4% |
Unknown | 6 | 13% |