Title |
Superoxide Generation and Its Involvement in the Growth of Mycobacterium smegmatis
|
---|---|
Published in |
Frontiers in Microbiology, January 2017
|
DOI | 10.3389/fmicb.2017.00105 |
Pubmed ID | |
Authors |
Amar M Yeware, Ketaki D Shurpali, Meghana C Athalye, Dhiman Sarkar |
Abstract |
Superoxide generation is inevitable in aerobic organisms, most of which have developed mechanisms to detoxify superoxides. However, its significance has not been clearly understood in mycobacteria. This study demonstrates that NADH oxidase is the major source of superoxide in Mycobacterium smegmatis and elucidates the involvement of superoxide in M. smegmatis growth. The maximum inhibition of superoxide generation was observed in the presence of diphenyleneiodonium chloride (DPI), an NADH oxidase inhibitor, compared to other standard inhibitors. After incubation for 24 h, the number of colony forming units (CFUs) was reduced by 6.8 log10 compared to the untreated culture. The inhibitory effect of DPI on M. smegmatis was reversed when the same culture was supplemented with menadione and pyrogallol, which are superoxide generators. Thus, this study reports the source of superoxide generation and its involvement in the growth of M. smegmatis. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 32% |
Student > Ph. D. Student | 2 | 9% |
Other | 1 | 5% |
Student > Master | 1 | 5% |
Student > Postgraduate | 1 | 5% |
Other | 0 | 0% |
Unknown | 10 | 45% |
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Biochemistry, Genetics and Molecular Biology | 4 | 18% |
Immunology and Microbiology | 2 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Physics and Astronomy | 1 | 5% |
Other | 0 | 0% |
Unknown | 10 | 45% |