Efficient T cell engineering for cancer therapy requires expressing CARs or TCRs. This study used #CRISPR/Cas9 to knockout B2M, TRAC, and PD1 genes and insert a GD2-CAR non-virally. These cells effectively targeted neuroblastoma. insights 👉🏼 @gathersight.
RT @ScienceCrisp: “Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells” Cappabianca D, Li J […
“Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells” Cappabianca D, Li J [..] Deans R, Saha K. Front Bioeng Biotechnol. 2024-05-31. https://t.co/k3JJfpwfrI #CRISPR-Cas9 KO https://t.co/Ewa0XVp2rk