Title |
A genetically-encoded chloride and pH sensor for dissociating ion dynamics in the nervous system
|
---|---|
Published in |
Frontiers in Cellular Neuroscience, January 2013
|
DOI | 10.3389/fncel.2013.00202 |
Pubmed ID | |
Authors |
Joseph V. Raimondo, Bradley Joyce, Louise Kay, Theresa Schlagheck, Sarah E. Newey, Shankar Srinivas, Colin J. Akerman |
Abstract |
Within the nervous system, intracellular Cl(-) and pH regulate fundamental processes including cell proliferation, metabolism, synaptic transmission, and network excitability. Cl(-) and pH are often co-regulated, and network activity results in the movement of both Cl(-) and H(+). Tools to accurately measure these ions are crucial for understanding their role under physiological and pathological conditions. Although genetically-encoded Cl(-) and pH sensors have been described previously, these either lack ion specificity or are unsuitable for neuronal use. Here we present ClopHensorN-a new genetically-encoded ratiometric Cl(-) and pH sensor that is optimized for the nervous system. We demonstrate the ability of ClopHensorN to dissociate and simultaneously quantify Cl(-) and H(+) concentrations under a variety of conditions. In addition, we establish the sensor's utility by characterizing activity-dependent ion dynamics in hippocampal neurons. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 2 | 1% |
United Kingdom | 2 | 1% |
France | 1 | <1% |
Germany | 1 | <1% |
Finland | 1 | <1% |
Denmark | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Unknown | 159 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 37 | 22% |
Researcher | 35 | 21% |
Student > Master | 20 | 12% |
Student > Bachelor | 16 | 9% |
Professor | 11 | 7% |
Other | 24 | 14% |
Unknown | 26 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 49 | 29% |
Neuroscience | 36 | 21% |
Biochemistry, Genetics and Molecular Biology | 21 | 12% |
Medicine and Dentistry | 15 | 9% |
Chemistry | 7 | 4% |
Other | 11 | 7% |
Unknown | 30 | 18% |