Title |
Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins
|
---|---|
Published in |
Frontiers in Plant Science, January 2013
|
DOI | 10.3389/fpls.2013.00461 |
Pubmed ID | |
Authors |
David B. Knaff, Roger B. Sutton |
Abstract |
Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 14% |
Researcher | 2 | 14% |
Student > Postgraduate | 2 | 14% |
Student > Master | 2 | 14% |
Student > Doctoral Student | 1 | 7% |
Other | 2 | 14% |
Unknown | 3 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 36% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Nursing and Health Professions | 1 | 7% |
Chemistry | 1 | 7% |
Unknown | 5 | 36% |