Title |
The Soil Bacterium Methylococcus capsulatus Bath Interacts with Human Dendritic Cells to Modulate Immune Function
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Published in |
Frontiers in Microbiology, February 2017
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DOI | 10.3389/fmicb.2017.00320 |
Pubmed ID | |
Authors |
Stine Indrelid, Charlotte Kleiveland, René Holst, Morten Jacobsen, Tor Lea |
Abstract |
The prevalence of inflammatory bowel disease (IBD) has increased in Western countries during the course of the twentieth century, and is evolving to be a global disease. Recently we showed that a bacterial meal of a non-commensal, non-pathogenic methanotrophic soil bacterium, Methylococcus capsulatus Bath prevents experimentally induced colitis in a murine model of IBD. The mechanism behind the effect has this far not been identified. Here, for the first time we show that M. capsulatus, a soil bacterium adheres specifically to human dendritic cells, influencing DC maturation, cytokine production, and subsequent T cell activation, proliferation and differentiation. We characterize the immune modulatory properties of M. capsulatus and compare its immunological properties to those of another Gram-negative gammaproteobacterium, the commensal Escherichia coli K12, and the immune modulatory Gram-positive probiotic bacterium, Lactobacillus rhamnosus GG in vitro. M. capsulatus induces intermediate phenotypic and functional DC maturation. In a mixed lymphocyte reaction M. capsulatus-primed monocyte-derived dendritic cells (MoDCs) enhance T cell expression of CD25, the γ-chain of the high affinity IL-2 receptor, supports cell proliferation, and induce a T cell cytokine profile different from both E. coli K12 and Lactobacillus rhamnosus GG. M. capsulatus Bath thus interacts specifically with MoDC, affecting MoDC maturation, cytokine profile, and subsequent MoDC directed T cell polarization. |
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Unknown | 47 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 21% |
Student > Ph. D. Student | 9 | 19% |
Other | 7 | 15% |
Student > Bachelor | 6 | 13% |
Student > Master | 4 | 9% |
Other | 2 | 4% |
Unknown | 9 | 19% |
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Economics, Econometrics and Finance | 1 | 2% |
Other | 2 | 4% |
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