Title |
Transgenic expression of the human LEDGF/p75 gene relieves the species barrier against HIV-1 infection in mouse cells
|
---|---|
Published in |
Frontiers in Microbiology, January 2013
|
DOI | 10.3389/fmicb.2013.00377 |
Pubmed ID | |
Authors |
Takuya Tada, Motohiko Kadoki, Yang Liu, Kenzo Tokunaga, Yoichiro Iwakura |
Abstract |
Attempts to create mouse models for AIDS have been hampered by species barriers in HIV-1 infection. We previously showed that the nuclear accumulation of HIV-1 preintegration complex (PIC) was suppressed in mouse cells. Lens epithelium-derived growth factor (LEDGF/p75) is a host factor identified as a binding partner of integrase (IN), and has been suggested to be involved in promoting viral integration by tethering PIC to the chromatin, which are observed as nuclear accumulation of IN by LEDGF/p75. Therefore, we here hypothesized that this host factor might act as one of the species-specific barriers in mouse cells. We generated transgenic (Tg) mice that constitutively express human (h) LEDGF/p75. The GFP-fused IN was efficiently accumulated into the nucleus of hLEDGF/p75 expressing Tg mouse embryonic fibroblast (MEF) cells in contrast to the control MEF cells. Importantly, hLEDGF/p75 Tg MEF cells were significantly more susceptible to HIV-1 infection. These results suggest that LEDGF/p75 is one of the host factors that constitute species barrier against HIV-1 in mouse cells. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 6% |
Unknown | 15 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 44% |
Student > Ph. D. Student | 2 | 13% |
Professor | 2 | 13% |
Other | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 38% |
Biochemistry, Genetics and Molecular Biology | 3 | 19% |
Immunology and Microbiology | 2 | 13% |
Business, Management and Accounting | 1 | 6% |
Chemistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 3 | 19% |