Title |
A simple and efficient total genomic DNA extraction method for individual zooplankton
|
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Published in |
SpringerPlus, December 2016
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DOI | 10.1186/s40064-016-3724-x |
Pubmed ID | |
Authors |
Hanafiah Fazhan, Khor Waiho, Md. Sheriff Shahreza |
Abstract |
Molecular approaches are widely applied in species identification and taxonomic studies of minute zooplankton. One of the most focused zooplankton nowadays is from Subclass Copepoda. Accurate species identification of all life stages of the generally small sized copepods through molecular analysis is important, especially in taxonomic and systematic assessment of harpacticoid copepod populations and to understand their dynamics within the marine community. However, total genomic DNA (TGDNA) extraction from individual harpacticoid copepods can be problematic due to their small size and epibenthic behavior. In this research, six TGDNA extraction methods done on individual harpacticoid copepods were compared. The first new simple, feasible, efficient and consistent TGDNA extraction method was designed and compared with the commercial kit and modified available TGDNA extraction methods. The newly described TGDNA extraction method, "Incubation in PCR buffer" method, yielded good and consistent results based on the high success rate of PCR amplification (82%) compared to other methods. Coupled with its relatively consistent and economical method the "Incubation in PCR buffer" method is highly recommended in the TGDNA extraction of other minute zooplankton species. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 64 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 13 | 20% |
Researcher | 11 | 17% |
Student > Bachelor | 6 | 9% |
Student > Ph. D. Student | 5 | 8% |
Student > Doctoral Student | 3 | 5% |
Other | 6 | 9% |
Unknown | 20 | 31% |
Readers by discipline | Count | As % |
---|---|---|
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Biochemistry, Genetics and Molecular Biology | 9 | 14% |
Environmental Science | 6 | 9% |
Immunology and Microbiology | 2 | 3% |
Medicine and Dentistry | 2 | 3% |
Other | 2 | 3% |
Unknown | 21 | 33% |