Tong Zhang, Changbin Gao, Yao Yue, Zhiquan Liu, Chaozhi Ma, Guilong Zhou, Yong Yang, Zhiqiang Duan, Bing Li, Jing Wen, Bin Yi, Jinxiong Shen, Jinxing Tu, Tingdong Fu
Abstract
Brassica species exhibit both compatible and incompatible pollen-stigma interactions, however, the underlying molecular mechanisms remain largely unknown. Here, RNA-seq technology was applied in a comprehensive time-course experiment (2, 5, 10, 20, and 30 min) to explore gene expression during compatible/incompatible pollen-stigma interactions in stigma. Moderate changes of gene expression were observed both in compatible pollination (PC) and incompatible pollination (PI) within 10 min, whereas drastic changes showed up by 30 min, especially in PI. Stage specific DEGs [Differentially Expressed Gene(s)] were identified, and signaling pathways such as stress response, defense response, cell wall modification and others were found to be over-represented. In addition, enriched genes in all samples were analyzed as well, 293 most highly expressed genes were identified and annotated. Gene Ontology and metabolic pathway analysis revealed 10 most highly expressed genes and 37 activated metabolic pathways. According to the data, downstream components were activated in signaling pathways of both compatible and incompatible responses, and incompatible response had more complicated signal transduction networks. This study provides more detailed molecular information at different time points after compatible and incompatible pollination, deepening our knowledge about pollen-stigma interactions.
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2017.
All research outputs
#15,459,782
of 22,973,051 outputs
Outputs from Frontiers in Plant Science
#10,960
of 20,410 outputs
Outputs of similar age
#194,889
of 310,926 outputs
Outputs of similar age from Frontiers in Plant Science
#374
of 597 outputs
Altmetric has tracked 22,973,051 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,410 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,926 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 597 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
