Title |
Aldehyde dehydrogenase activity identifies a subpopulation of canine adipose-derived stem cells with higher differentiation potential
|
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Published in |
Journal of Veterinary Medical Science, June 2017
|
DOI | 10.1292/jvms.16-0503 |
Pubmed ID | |
Authors |
Harumichi Itoh, Shimpei Nishikawa, Tomoya Haraguchi, Yu Arikawa, Masato Hiyama, Shotaro Eto, Toshie Iseri, Yoshiki Itoh, Kenji Tani, Munekazu Nakaichi, Yasuho Taura, Kazuhito Itamoto |
Abstract |
Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activity-a widely used stem cell marker in mice and humans-by flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity. Compared to cells with low activity (ALDH(Lo)), the high-activity (ALDH(Hi)) subpopulation exhibited a higher capacity for adipogenic and osteogenic differentiation. This is the first report of distinct ADSC subpopulations in dogs that differ in terms of adipogenic and osteogenic differentiation potential. |
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Unknown | 2 | 100% |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 38% |
Student > Doctoral Student | 2 | 25% |
Student > Bachelor | 1 | 13% |
Librarian | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 1 | 13% |
Social Sciences | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Engineering | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |