Title |
Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria
|
---|---|
Published in |
Frontiers in Microbiology, June 2017
|
DOI | 10.3389/fmicb.2017.01161 |
Pubmed ID | |
Authors |
Lieke A. van Gijtenbeek, Jan Kok |
Abstract |
To be able to visualize the abundance and spatiotemporal features of RNAs in bacterial cells would permit obtaining a pivotal understanding of many mechanisms underlying bacterial cell biology. The first methods that allowed observing single mRNA molecules in individual cells were introduced by Bertrand et al. (1998) and Femino et al. (1998). Since then, a plethora of techniques to image RNA molecules with the aid of fluorescence microscopy has emerged. Many of these approaches are useful for the large eukaryotic cells but their adaptation to study RNA, specifically mRNA molecules, in bacterial cells progressed relatively slow. Here, an overview will be given of fluorescent techniques that can be used to reveal specific RNA molecules inside fixed and living single bacterial cells. It includes a critical evaluation of their caveats as well as potential solutions. |
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Country | Count | As % |
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United States | 5 | 24% |
United Kingdom | 2 | 10% |
Denmark | 2 | 10% |
Switzerland | 1 | 5% |
France | 1 | 5% |
Netherlands | 1 | 5% |
Spain | 1 | 5% |
Unknown | 8 | 38% |
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Scientists | 16 | 76% |
Members of the public | 5 | 24% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
Unknown | 98 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 30 | 30% |
Researcher | 15 | 15% |
Student > Master | 8 | 8% |
Student > Bachelor | 5 | 5% |
Student > Doctoral Student | 4 | 4% |
Other | 10 | 10% |
Unknown | 27 | 27% |
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Agricultural and Biological Sciences | 24 | 24% |
Chemistry | 5 | 5% |
Immunology and Microbiology | 4 | 4% |
Physics and Astronomy | 3 | 3% |
Other | 8 | 8% |
Unknown | 26 | 26% |