Title |
An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
|
---|---|
Published in |
Frontiers in Plant Science, June 2017
|
DOI | 10.3389/fpls.2017.01033 |
Pubmed ID | |
Authors |
María L. Giachero, Nathalie Marquez, Adrien Gallou, Celina M. Luna, Stéphane Declerck, Daniel A. Ducasse |
Abstract |
In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 68 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 15 | 22% |
Student > Ph. D. Student | 14 | 21% |
Student > Bachelor | 5 | 7% |
Student > Master | 5 | 7% |
Student > Postgraduate | 4 | 6% |
Other | 7 | 10% |
Unknown | 18 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 35 | 51% |
Biochemistry, Genetics and Molecular Biology | 4 | 6% |
Immunology and Microbiology | 2 | 3% |
Unspecified | 1 | 1% |
Arts and Humanities | 1 | 1% |
Other | 3 | 4% |
Unknown | 22 | 32% |