Title |
Oral Administration of a Seed-based Bivalent Rotavirus Vaccine Containing VP6 and NSP4 Induces Specific Immune Responses in Mice
|
---|---|
Published in |
Frontiers in Plant Science, May 2017
|
DOI | 10.3389/fpls.2017.00910 |
Pubmed ID | |
Authors |
Hao Feng, Xin Li, Weibin Song, Mei Duan, Hong Chen, Tao Wang, Jiangli Dong |
Abstract |
Rotavirus is the leading cause of severe diarrheal disease among newborns. Plant-based rotavirus vaccines have been developed in recent years and have been proven to be effective in animal models. In the present study, we report a bivalent vaccine candidate expressing rotavirus subunits VP6 and NSP4 fused with the adjuvant subunit B of E. coli heat-labile enterotoxin (LTB) in maize seeds. The RT-PCR and Western blot results showed that VP6 and LTB-NSP4 antigens were expressed and accumulated in maize seeds. The expression levels were as high as 0.35 and 0.20% of the total soluble protein for VP6 and LTB-NSP4, respectively. Oral administration of transgenic maize seeds successfully stimulated systemic and mucosal responses, with high titers of serum IgG and mucosal IgA antibodies, even after long-term storage. This study is the first to use maize seeds as efficient generators for the development of a bivalent vaccine against rotavirus. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 3 | 12% |
Student > Bachelor | 3 | 12% |
Student > Master | 3 | 12% |
Student > Ph. D. Student | 3 | 12% |
Researcher | 2 | 8% |
Other | 2 | 8% |
Unknown | 10 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 15% |
Biochemistry, Genetics and Molecular Biology | 3 | 12% |
Medicine and Dentistry | 3 | 12% |
Immunology and Microbiology | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 3 | 12% |
Unknown | 10 | 38% |