Title |
Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
|
---|---|
Published in |
International Journal of Nanomedicine, January 2017
|
DOI | 10.2147/ijn.s121948 |
Pubmed ID | |
Authors |
Chenhui Wang, Jiebing Yang, Haobo Han, Jiawen Chen, Yudi Wang, Quanshun Li, Yanbo Wang |
Abstract |
In this study, poly(lactic-co-glycolic acid) (PLGA) was used as a carrier to construct disulfiram-loaded porous microparticle through the emulsion solvent evaporation method, using ammonium bicarbonate as a porogen. The microparticle possessed highly porous surface, suitable aerodynamic diameter for inhalation (8.31±1.33 µm), favorable drug loading (4.09%±0.11%), and sustained release profile. The antiproliferation effect of release supernatant was detected through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using non-small-cell lung cancer A549 as a model, with only 13.3% of cell viability observed for the release supernatant at 7 days. The antiproliferation mechanism was elucidated to be associated with the enhanced induction of cell apoptosis and cell cycle arrest at S phase through flow cytometry and Western blotting analysis. Finally, wound healing and transwell migration assay showed that they could efficiently inhibit the cell migration. These results demonstrated that disulfiram-loaded porous PLGA microparticle could achieve favorable antitumor efficiency, implying the potential of treating non-small-cell lung cancer in a pulmonary administration. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 23% |
Student > Bachelor | 5 | 14% |
Researcher | 4 | 11% |
Student > Master | 4 | 11% |
Other | 2 | 6% |
Other | 6 | 17% |
Unknown | 6 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 9 | 26% |
Biochemistry, Genetics and Molecular Biology | 5 | 14% |
Medicine and Dentistry | 3 | 9% |
Chemistry | 3 | 9% |
Sports and Recreations | 1 | 3% |
Other | 5 | 14% |
Unknown | 9 | 26% |