Title |
Changes in the Total Fecal Bacterial Population in Individual Horses Maintained on a Restricted Diet Over 6 Weeks
|
---|---|
Published in |
Frontiers in Microbiology, August 2017
|
DOI | 10.3389/fmicb.2017.01502 |
Pubmed ID | |
Authors |
Kirsty Dougal, Patricia A. Harris, Susan E. Girdwood, Christopher J. Creevey, Gemma C. Curtis, Clare F. Barfoot, Caroline M. Argo, Charles J. Newbold |
Abstract |
Twelve mature (aged 5-16 years) horses and ponies of mixed breed and type were fed restricted (1.25% BM Dry matter) quantities of one of two fiber based diets formulated to be iso-caloric. Diet 1 comprised of 0.8% body mass (BM) of chaff based complete feed plus 0.45% BM low energy grass hay (the same hay used for both diets). Diet 2 comprised 0.1% BM of a nutrient balancer plus 1.15% BM grass hay. Fecal samples were collected at week 10 and week 16. DNA was extracted and the V1-V2 regions of 16SrDNA were 454-pyrosequenced to investigate the bacterial microbiome of the horse. The two most abundant phyla found in both diets and sampling periods were the Firmicutes and Bacteroidetes. There was a clear reduction in Bacteroidetes with a concordant increase in Firmicutes over time. There was a limited degree of stability within the bacterial community of the hindgut of horses, with 65% of bacteria retained, over a 6 week period whilst on a uniform diet. The presence of a core community defined by being present in all samples (each animal/diet combination) included in the study and being present at 0.1% relative abundance (or greater) was identified. In total 65 operational taxonomic units (OTUs) were identified that fit the definition of core making up 21-28% of the total sequences recovered. As with total population the most abundant phyla were the Bacteroidetes followed by the Firmicutes, however there was no obvious shift in phyla due to period. Indeed, when the relative abundance of OTUs was examined across diets and periods there was no significant effect of diet or period alone or in combination on the relative abundance of the core OTUs. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 62 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 16% |
Researcher | 8 | 13% |
Unspecified | 7 | 11% |
Student > Doctoral Student | 5 | 8% |
Student > Master | 5 | 8% |
Other | 14 | 23% |
Unknown | 13 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 29% |
Veterinary Science and Veterinary Medicine | 11 | 18% |
Unspecified | 7 | 11% |
Medicine and Dentistry | 3 | 5% |
Environmental Science | 2 | 3% |
Other | 5 | 8% |
Unknown | 16 | 26% |