Title |
VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
|
---|---|
Published in |
International Journal of Nanomedicine, August 2017
|
DOI | 10.2147/ijn.s142739 |
Pubmed ID | |
Authors |
Jinliang Chen, Xiaoyi Sun, Rong Shao, Yichao Xu, Jianqing Gao, Wenquan Liang |
Abstract |
Angiogenesis plays an important role in tumor development and metastasis, and many cancer cells upregulate VEGF expression to promote angiogenesis. Silencing VEGF expression by RNA interference is expected to be a promising strategy to suppress the tumor growth. However, low transfection efficiency and instability are the main barriers for small interfering RNA (siRNA) delivery. In this study, we developed polycation liposome-encapsulated calcium phosphate nanoparticles (PLCP) for siRNA delivery in vivo. VEGF expression silencing effect in MCF-7 cells was investigated by real-time quantitative polymerase chain reaction and Western blot assay. VEGF siRNA mediated by PLCP can reduce 60%-80% VEGF expression in vitro, which was significantly higher than that mediated by Lipofectamine 2000. Furthermore, significant tumor growth and angiogenesis inhibition were observed in MCF-7 xenografts mice when treated with PLCP/VEGF siRNA or combined with doxorubicin. In conclusion, the combination of silencing VEGF expression and chemotherapeutics would be a potential treatment for cancer therapy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 16% |
Researcher | 5 | 12% |
Student > Doctoral Student | 4 | 9% |
Student > Master | 4 | 9% |
Professor > Associate Professor | 3 | 7% |
Other | 5 | 12% |
Unknown | 15 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 7 | 16% |
Chemistry | 5 | 12% |
Biochemistry, Genetics and Molecular Biology | 3 | 7% |
Engineering | 3 | 7% |
Agricultural and Biological Sciences | 2 | 5% |
Other | 6 | 14% |
Unknown | 17 | 40% |