Title |
A Chemical-Induced, Seed-Soaking Activation Procedure for Regulated Gene Expression in Rice
|
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Published in |
Frontiers in Plant Science, August 2017
|
DOI | 10.3389/fpls.2017.01447 |
Pubmed ID | |
Authors |
Zaijie Chen, Qianqian Cheng, Chanquan Hu, Xinrui Guo, Ziqiang Chen, Yan Lin, Taijiao Hu, Maria Bellizzi, Guodong Lu, Guo-Liang Wang, Zonghua Wang, Songbiao Chen, Feng Wang |
Abstract |
Inducible gene expression has emerged as a powerful tool for plant functional genomics. The estrogen receptor-based, chemical-inducible system XVE has been used in many plant species, but the limited systemic movement of inducer β-estradiol in transgenic rice plants has prohibited a wide use of the XVE system in this important food crop. Here, we constructed an improved chemical-regulated, site-specific recombination system by employing the XVE transactivator in combination with a Cre/loxP-FRT system, and optimized a seed-soaking procedure for XVE induction in rice. By using a gus gene and an hpRNAi cassette targeted for OsPDS as reporters, we demonstrated that soaking transgenic seeds with estradiol solution could induce highly efficient site-specific recombination in germinating embryos, resulting in constitutive and high-level expression of target gene or RNAi cassette in intact rice plants from induced seeds. The strategy reported here thereby provides a useful gene activation approach for effectively regulating gene expression in rice. |
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