Fabrício A. Voltarelli, Fernando T. Frajacomo, Camila de Souza Padilha, Mayra T. J. Testa, Paola S. Cella, Diogo F. Ribeiro, Donizete X. de Oliveira, Luciana C. Veronez, Gabriela S. Bisson, Felipe A. Moura, Rafael Deminice
Abstract
Muscle wasting has been emerging as one of the principal components of cancer cachexia, leading to progressive impairment of work capacity. Despite early stages melanomas rarely promotes weight loss, the appearance of metastatic and/or solid tumor melanoma can leads to cachexia development. Here, we investigated the B16F10 tumor-induced cachexia and its contribution to muscle strength and locomotor-like activity impairment. C57BL/6 mice were subcutaneously injected with 5 × 10(4) B16F10 melanoma cells or PBS as a Sham negative control. Tumor growth was monitored during a period of 28 days. Compared to Sham mice, tumor group depicts a loss of skeletal muscle, as well as significantly reduced muscle grip strength and epididymal fat mass. This data are in agreement with mild to severe catabolic host response promoted by elevated serum tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and lactate dehydrogenase (LDH) activity. Tumor implantation has also compromised general locomotor activity and decreased exploratory behavior. Likewise, muscle loss, and elevated inflammatory interleukin were associated to muscle strength loss and locomotor activity impairment. In conclusion, our data demonstrated that subcutaneous B16F10 melanoma tumor-driven catabolic state in response to a pro-inflammatory environment that is associated with impaired skeletal muscle strength and decreased locomotor activity in tumor-bearing mice.
Pharmacology, Toxicology and Pharmaceutical Science
6
7%
Immunology and Microbiology
4
5%
Other
13
15%
Unknown
23
26%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 October 2017.
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#20,449,496
of 23,005,189 outputs
Outputs from Frontiers in Physiology
#9,475
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#280,248
of 321,103 outputs
Outputs of similar age from Frontiers in Physiology
#226
of 315 outputs
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