Title |
Identification of a set of endogenous reference genes for miRNA expression studies in Parkinson’s disease blood samples
|
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Published in |
BMC Research Notes, October 2014
|
DOI | 10.1186/1756-0500-7-715 |
Pubmed ID | |
Authors |
Alice Serafin, Luisa Foco, Hagen Blankenburg, Anne Picard, Stefano Zanigni, Alessandra Zanon, Peter P Pramstaller, Andrew A Hicks, Christine Schwienbacher |
Abstract |
Research on microRNAs (miRNAs) is becoming an increasingly attractive field, as these small RNA molecules are involved in several physiological functions and diseases. To date, only few studies have assessed the expression of blood miRNAs related to Parkinson's disease (PD) using microarray and quantitative real-time PCR (qRT-PCR). Measuring miRNA expression involves normalization of qRT-PCR data using endogenous reference genes for calibration, but their choice remains a delicate problem with serious impact on the resulting expression levels. The aim of the present study was to evaluate the suitability of a set of commonly used small RNAs as normalizers and to identify which of these miRNAs might be considered reliable reference genes in qRT-PCR expression analyses on PD blood samples. |
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