Title |
A Linear 19-Mer Plant Defensin-Derived Peptide Acts Synergistically with Caspofungin against Candida albicans Biofilms
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Published in |
Frontiers in Microbiology, October 2017
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DOI | 10.3389/fmicb.2017.02051 |
Pubmed ID | |
Authors |
Tanne L. Cools, Caroline Struyfs, Jan W. Drijfhout, Soňa Kucharíková, Celia Lobo Romero, Patrick Van Dijck, Marcelo H. S. Ramada, Carlos Bloch, Bruno P. A. Cammue, Karin Thevissen |
Abstract |
Public health problems are associated with device-associated biofilm infections, with Candida albicans being the major fungal pathogen. We previously identified potent antibiofilm combination treatment in which the antifungal plant defensin HsAFP1 is co-administered with caspofungin, the preferred antimycotic to treat such infections. In this study, we identified the smallest linear HsAFP1-derived peptide that acts synergistically with caspofungin or anidulafungin against C. albicans as HsLin06_18, a 19-mer peptide derived from the C-terminal part of HsAFP1. The [caspofungin + HsLin06_18] combination significantly reduced in vitro biofilm formation of Candida glabrata and C. albicans on catheters, as well as biofilm formation of a caspofungin-resistant C. albicans strain. The [caspofungin + HsLin06_18] combination was not cytotoxic and reduced biofilm formation of C. albicans in vivo using a subcutaneous rat catheter model, as compared to control treatment. Mode of action research on the [caspofungin + HsLin06_18] combination pointed to caspofungin-facilitated HsLin06_18 internalization and immediate membrane permeabilization. All these findings point to broad-spectrum antibiofilm activity of a combination of HsLin06_18 and caspofungin. |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 51 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 7 | 14% |
Student > Ph. D. Student | 6 | 12% |
Professor | 5 | 10% |
Other | 5 | 10% |
Student > Bachelor | 3 | 6% |
Other | 10 | 20% |
Unknown | 15 | 29% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Immunology and Microbiology | 7 | 14% |
Agricultural and Biological Sciences | 6 | 12% |
Chemistry | 5 | 10% |
Medicine and Dentistry | 4 | 8% |
Other | 7 | 14% |
Unknown | 14 | 27% |