Title |
Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, November 2017
|
DOI | 10.3389/fcimb.2017.00475 |
Pubmed ID | |
Authors |
Kendal G. Cooper, Audrey Chong, Tregei Starr, Ciaran E. Finn, Olivia Steele-Mortimer |
Abstract |
Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined with a synthetic transcriptional terminator, and plasmid copy number variation. This yielded a series of plasmids that drive uniform production of fluorescent and endogenous proteins, over a wide dynamic range. We describe several examples where this system is used to fine-tune constitutive expression in Salmonella, providing an efficient means to titrate out toxic effects of protein production. |
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Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 40% |
United Kingdom | 1 | 20% |
Canada | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 60% |
Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Members of the public | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 58 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 15 | 26% |
Student > Ph. D. Student | 10 | 17% |
Researcher | 6 | 10% |
Student > Bachelor | 5 | 9% |
Professor > Associate Professor | 3 | 5% |
Other | 4 | 7% |
Unknown | 15 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 18 | 31% |
Agricultural and Biological Sciences | 9 | 16% |
Immunology and Microbiology | 7 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Social Sciences | 1 | 2% |
Other | 1 | 2% |
Unknown | 21 | 36% |