Title |
D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design
|
---|---|
Published in |
Frontiers in Microbiology, November 2014
|
DOI | 10.3389/fmicb.2014.00608 |
Pubmed ID | |
Authors |
Pei-Fang Hsieh, Meng-Chuan Wu, Feng-Ling Yang, Chun-Tang Chen, Tzu-Chi Lou, Yi-Yin Chen, Shih-Hsiung Wu, Jin-Chuan Sheu, Jin-Town Wang |
Abstract |
In the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galactan II in bacterial pathogenesis remain unclear. Here, we report the identification of the D-galactan II-synthesizing genes by screening a transposon mutant library of an acapsulated Klebsiella pneumoniae O1 strain with bacteriophage. K. pneumoniae strain deleted for wbbY exhibited abrogated D-galactan II production; altered serum resistance and attenuation of virulence. Serologic analysis of K. pneumoniae clinical isolates demonstrated that D-galactan II was more prevalent in community-acquired pyogenic liver abscess (PLA)-causing strains than in non-tissue-invasive strains. WbbY homologs, WbbZ homologs, and lipopolysaccharide structures based on D-galactan II also were present in several Gram-negative bacteria. Immunization of mice with the magA-mutant (K(-) 1 O1) (that is, with a LPS D-galactan II-producing strain) provided protection against infection with an O1:K2 PLA strain. Our findings indicate that both WbbY and WbbZ homologs are sufficient for the synthesis of D-galactan II. D-galactan II represents an immunodominant antigen; is conserved among multiple species of Gram-negative bacteria and could be a useful vaccine candidate. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 40 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 8 | 20% |
Student > Ph. D. Student | 7 | 18% |
Researcher | 7 | 18% |
Student > Master | 5 | 13% |
Student > Doctoral Student | 2 | 5% |
Other | 4 | 10% |
Unknown | 7 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 9 | 23% |
Agricultural and Biological Sciences | 8 | 20% |
Biochemistry, Genetics and Molecular Biology | 7 | 18% |
Unspecified | 2 | 5% |
Medicine and Dentistry | 2 | 5% |
Other | 4 | 10% |
Unknown | 8 | 20% |