Chapter title |
GC-MS Analysis of Short-Chain Fatty Acids in Feces, Cecum Content, and Blood Samples
|
---|---|
Chapter number | 17 |
Book title |
Clinical Metabolomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7592-1_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7591-4, 978-1-4939-7592-1
|
Authors |
Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, Martin Giera |
Abstract |
Short-chain fatty acids, the end products of fermentation of dietary fibers by the gut microbiota, have been shown to exert multiple effects on mammalian metabolism. For the analysis of short-chain fatty acids, gas chromatography-mass spectrometry is a very powerful and reliable method. Here, we describe a fast, reliable, and reproducible method for the separation and quantification of short-chain fatty acids in mouse feces, cecum content, and blood samples (i.e., plasma or serum) using gas chromatography-mass spectrometry. The short-chain fatty acids analyzed include acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, and heptanoic acid. |
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Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 165 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 26 | 16% |
Student > Master | 24 | 15% |
Researcher | 20 | 12% |
Student > Bachelor | 18 | 11% |
Other | 7 | 4% |
Other | 18 | 11% |
Unknown | 52 | 32% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 27 | 16% |
Agricultural and Biological Sciences | 19 | 12% |
Chemistry | 13 | 8% |
Medicine and Dentistry | 12 | 7% |
Immunology and Microbiology | 10 | 6% |
Other | 23 | 14% |
Unknown | 61 | 37% |