Clinical Metabolomics
Humana Press, New York, NY
Chapter title |
Shotgun Lipidomics Approach for Clinical Samples
|
---|---|
Chapter number | 12 |
Book title |
Clinical Metabolomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7592-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7591-4, 978-1-4939-7592-1
|
Authors |
Lars F. Eggers, Dominik Schwudke |
Abstract |
Shotgun lipidomics offers fast and reproducible identification and quantification of lipids in clinical samples. Lipid extraction procedures based on the methyl tert-butyl protocol are well established for performing shotgun lipidomics in biomedical research. Here, we describe a shotgun lipidomics workflow that is well suited for the analysis of clinical samples such as tissue samples, blood plasma, and peripheral blood mononuclear cells. |
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