Title |
Cellular Tight Junctions Prevent Effective Campylobacter jejuni Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells
|
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Published in |
Frontiers in Cellular and Infection Microbiology, January 2018
|
DOI | 10.3389/fcimb.2018.00015 |
Pubmed ID | |
Authors |
Sho Hatayama, Takaaki Shimohata, Sachie Amano, Junko Kido, Anh Q. Nguyen, Yuri Sato, Yuna Kanda, Aya Tentaku, Shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi |
Abstract |
Campylobacter jejuni invasion is closely related toC. jejunipathogenicity. The intestinal epithelium contains polarized epithelial cells that form tight junctions (TJs) to provide a physical barrier against bacterial invasion. Previous studies indicated thatC. jejuniinvasion of non-polarized cells involves several cellular features, including lipid rafts. However, the dynamics ofC. jejuniinvasion of polarized epithelial cells are not fully understood. Here we investigated the interaction betweenC. jejuniinvasion and TJ formation to characterize the mechanism ofC. jejuniinvasion in polarized epithelial cells. In contrast to non-polarized epithelial cells,C. jejuniinvasion was not affected by depletion of lipid rafts in polarized epithelial cells. However, depletion of lipid rafts significantly decreasedC. jejuniinvasion in TJ disrupted cells or basolateral infection and repair of cellular TJs suppressed lipid raft-mediatedC. jejuniinvasion in polarized epithelial cells. In addition, pro-inflammatory cytokine, TNF-α treatment that induce TJ disruption promoteC. jejuniinvasion and lipid rafts depletion significantly reducedC. jejuniinvasion in TNF-α treated cells. These data demonstrated that TJs preventC. jejuniinvasion from the lateral side of epithelial cells, where they play a main part in bacterial invasion and suggest thatC. jejuniinvasion could be increased in inflammatory condition. Therefore, maintenance of TJs integrity should be considered important in the development of novel therapies forC. jejuniinfection. |
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