Title |
Mutation of Agr Is Associated with the Adaptation of Staphylococcus aureus to the Host during Chronic Osteomyelitis
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, February 2018
|
DOI | 10.3389/fcimb.2018.00018 |
Pubmed ID | |
Authors |
Carlos M. Suligoy, Santiago M. Lattar, Mariángeles Noto Llana, Cintia D. González, Lucía P. Alvarez, D. Ashley Robinson, Marisa I. Gómez, Fernanda R. Buzzola, Daniel O. Sordelli |
Abstract |
Selection pressures exerted onStaphylococcus aureusby host factors may lead to the emergence of mutants better adapted to the evolving conditions at the infection site. This study was aimed at identifying the changes that occur inS. aureusexposed to the host defense mechanisms during chronic osteomyelitis and evaluating whether these changes affect the virulence of the organism. Genome assessment of twoS. aureusisolates collected 13 months apart (HU-85a and HU-85c) from a host with chronic osteomyelitis was made by whole genome sequencing. Agr functionality was assessed by qRT-PCR. Isolates were tested in a rat model of osteomyelitis and the bacterial load (CFU/tibia) and the morphometric osteomyelitic index (OI) were determined. The ability of the isolates to trigger the release of proinflammatory cytokines was determined on macrophages in culture. Persistence ofS. aureuswithin the host resulted in anagrCframeshift mutation that likely led to the observed phenotype. The capacity to cause bone tissue damage and trigger proinflammatory cytokines by macrophages of theagr-deficient, unencapsulated derivative (HU-85c) was decreased when compared with those of the isogenic CP8-capsulated parental strain (HU-85a). By comparison, no significant differences were found in the bacterial load or the OI from rats challenged with isogenic Reynolds strains [CP5, CP8, and non-typeable (NT)], indicating that lack of CP expression alone was not likely responsible for the reduced capacity to cause tissue damage in HU-85c compared with HU-85a. The production of biofilm was significantly increased in the isogenic derivative HU-85c. Lack ofagr-dependent factors makesS. aureusless virulent during chronic osteomyelitis and alteration of theagrfunctionality seems to permit better adaptation ofS. aureusto the chronically infected host. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 25% |
Sweden | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 73 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 21% |
Student > Bachelor | 13 | 18% |
Researcher | 10 | 14% |
Student > Doctoral Student | 6 | 8% |
Student > Master | 5 | 7% |
Other | 9 | 12% |
Unknown | 15 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 20 | 27% |
Immunology and Microbiology | 19 | 26% |
Medicine and Dentistry | 5 | 7% |
Agricultural and Biological Sciences | 4 | 5% |
Engineering | 3 | 4% |
Other | 6 | 8% |
Unknown | 16 | 22% |