Xiao-Dong Zhang, Chun-Xia He, Jun Cheng, Jing Wen, Peng-Yun Li, Na Wang, Guang Li, Xiao-Rong Zeng, Ji-Min Cao, Yan Yang
Abstract
Background:
We previously have proved that sodium tanshinone II-A sulfonate (DS-201), a derivative of traditional Chinese medicinal herb Danshen (Salvia miltiorrhiza), is an opener and vasodilator of BKCachannel in the vascular smooth muscle cells (VSMCs). Vascular tension is closely associated with Ca2+dynamics and activation of BKCachannel may not be the sole mechanism for the relaxation of the vascular tension by DS-201. Therefore, we hypothesized that the vasorelaxing effect of DS-20 may be also related to Ca2+channel and cytoplasmic Ca2+level in the VSMCs.Methods:Arterial tension was measured by Danish Myo Technology (DMT) myograph system in the mesentery vessels of rats, intracellular Ca2+level by fluorescence imaging system in the VSMCs of rats, and L-type Ca2+current by patch clamp technique in Ca2+channels transfected human embryonic kidney 293 (HEK-293) cells.Results:DS-201 relaxed the endothelium-denuded artery rings pre-constricted with PE or high K+and the vasorelaxation was reversible. Blockade of K+channel did not totally block the effect of DS-201 on vasorelaxation. DS-201 suppressed [Ca2+]itransient induced by high K+in a concentration-dependent manner in the VSMCs, including the amplitude of Ca2+transient, the time for Ca2+transient reaching to the [Ca2+]ipeak and the time to remove Ca2+from the cytoplasm. DS-201 inhibited L-type Ca2+channel with an EC50of 59.5 μM and at about 40% efficacy of inhibition. However, DS-201did not significantly affect the kinetics of Ca2+channel. The effect of DS-201 on L-type Ca2+channel was rate-independent.Conclusion:The effect of DS-201 on vasorelaxation was not only via activating BKCachannel, but also blocking Ca2+channel and inhibiting Ca2+influx in the VSMCs of rats. The results favor the use of DS-201 and Danshen in the treatment of cardiovascular diseases clinically.
Pharmacology, Toxicology and Pharmaceutical Science
1
14%
Immunology and Microbiology
1
14%
Nursing and Health Professions
1
14%
Unknown
2
29%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 October 2018.
All research outputs
#12,868,847
of 23,020,670 outputs
Outputs from Frontiers in Pharmacology
#3,505
of 16,332 outputs
Outputs of similar age
#201,349
of 439,370 outputs
Outputs of similar age from Frontiers in Pharmacology
#68
of 293 outputs
Altmetric has tracked 23,020,670 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 16,332 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 439,370 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 293 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.
