Title |
The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ
|
---|---|
Published in |
Frontiers in Microbiology, February 2018
|
DOI | 10.3389/fmicb.2018.00238 |
Pubmed ID | |
Authors |
Marilena Falcone, Silvia Ferrara, Elio Rossi, Helle K. Johansen, Søren Molin, Giovanni Bertoni |
Abstract |
The small RNA ErsA ofPseudomonas aeruginosawas previously suggested to be involved in biofilm formation via negative post-transcriptional regulation of thealgCgene that encodes the virulence-associated enzyme AlgC, which provides sugar precursors for the synthesis of several polysaccharides. In this study, we show that a knock-outersAmutant strain forms a flat and uniform biofilm, not characterized by mushroom-multicellular structures typical of a mature biofilm. Conversely, the knock-out mutant strain showed enhanced swarming and twitching motilities. To assess the influence of ErsA on theP. aeruginosatranscriptome, we performed RNA-seq experiments comparing the knock-out mutant with the wild-type. More than 160 genes were found differentially expressed in the knock-out mutant. Parts of these genes, important for biofilm formation and motility regulation, are known to belong also to the AmrZ transcriptional regulator regulon. Here, we show that ErsA bindsin vitroand positively regulatesamrZmRNA at post-transcriptional levelin vivosuggesting an interesting contribution of the ErsA-amrZmRNA interaction in biofilm development at several regulatory levels. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 12% |
Sweden | 1 | 6% |
Italy | 1 | 6% |
Unknown | 13 | 76% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 9 | 53% |
Members of the public | 8 | 47% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 60 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 22% |
Student > Bachelor | 8 | 13% |
Student > Master | 6 | 10% |
Researcher | 5 | 8% |
Student > Doctoral Student | 3 | 5% |
Other | 6 | 10% |
Unknown | 19 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 18 | 30% |
Immunology and Microbiology | 11 | 18% |
Agricultural and Biological Sciences | 7 | 12% |
Computer Science | 1 | 2% |
Environmental Science | 1 | 2% |
Other | 2 | 3% |
Unknown | 20 | 33% |