Márcia Fernanda Correia Jardim Paz, Marcus Vinícius Oliveira Barros de Alencar, Antonio Luiz Gomes, Keylla da Conceição Machado, Muhammad Torequl Islam, Eunus S. Ali, Manik Chandra Shill, Iqbal Ahmed, Jamal Uddin, Ana Maria Oliveira Ferreira da Mata, Ricardo Melo de Carvalho, Kátia da Conceição Machado, André Luiz Pinho Sobral, Felipe Cavalcanti Carneiro da Silva, João Marcelo de Castro e Souza, Daniel Dias Rufino Arcanjo, Paulo Michel Pinheiro Ferreira, Siddhartha Kumar Mishra, Juliana da Silva, Ana Amélia de Carvalho Melo-Cavalcante
Abstract
Molecular epidemiological studies have identified several risk factors linking to the genes and external factors in the pathogenesis of breast cancer. In this sense, genetic instability caused by DNA damage and DNA repair inefficiencies are important molecular events for the diagnosis and prognosis of therapies. Therefore, the objective of this study was to analyze correlation between sociocultural, occupational, and lifestyle risk factors with levels of genetic instability in non-neoplastic cells of breast cancer patients. Total 150 individuals were included in the study that included 50 breast cancer patients submitted to chemotherapy (QT), 50 breast cancer patients submitted to radiotherapy (RT), and 50 healthy women without any cancer. Cytogenetic biomarkers for apoptosis and DNA damage were evaluated in samples of buccal epithelial and peripheral blood cells through micronuclei and comet assay tests. Elder age patients (61-80 years) had higher levels of apoptosis (catriolysis by karyolysis) and DNA damage at the diagnosis (baseline damage) with increased cell damage during QT and especially during RT. We also reported the increased frequencies of cytogenetic biomarkers in patients who were exposed to ionizing radiation as well as for alcoholism and smoking. QT and RT induced high levels of fragmentation (karyorrhexis) and nuclear dissolution (karyolysis) and DNA damage. Correlations were observed between age and karyorrhexis at diagnosis; smoking and karyolysis during RT; and radiation and karyolysis during QT. These correlations indicate that risk factors may also influence the genetic instability in non-neoplastic cells caused to the patients during cancer therapies.
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2019.
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