Fariza Abrão, Jessica A. Alves, Gessica Andrade, Pollyanna F. de Oliveira, Sérgio R. Ambrósio, Rodrigo C. S. Veneziani, Denise C. Tavares, Jairo K. Bastos, Carlos H. G. Martins
Abstract
This study evaluates the antibacterial activity of theCopaifera duckeiDwyer oleoresin and two isolated compounds [eperu-8(20)-15,18-dioic acid and polyalthic acid] against bacteria involved in primary endodontic infections and dental caries and assesses the cytotoxic effect of these substances against a normal cell line. MIC and MBC assays pointed out the most promising metabolites for further studies on bactericidal kinetics, antibiofilm activity, and synergistic antibacterial action. The oleoresin and polyalthic acid but not eperu-8(20)-15,18-dioic provided encouraging MIC and MBC results at concentrations lower than 100 μg mL-1. The oleoresin and polyalthic acid activities depended on the evaluated strain. A bactericidal effect onLactobacillus casei(ATCC 11578 and clinical isolate) emerged before 8 h of incubation. For all the tested bacteria, the oleoresin and polyalthic acid inhibited biofilm formation by at least 50%. The oleoresin and polyalthic acid gave the best activity againstActinomyces naeslundii(ATCC 19039) andL. casei(ATCC 11578), respectively. The synergistic assays combining the oleoresin or polyalthic acid with chlorhexidine did not afford interesting results. We examined the cytotoxicity ofC. duckeioleoresin, eperu-8(20)-15,18-dioic acid, and polyalthic acid against Chinese hamster lung fibroblasts. The oleoresin and polyalthic acid were cytotoxic at concentrations above 78.1 μg mL-1, whereas eperu-8(20)-15,18-dioic displayed cytotoxicity at concentrations above 312.5 μg mL-1. In conclusion, the oleoresin and polyalthic acid are potential sources of antibacterial agents against bacteria involved in primary endodontic infections and dental caries in both the sessile and the planktonic modes at concentrations that do not cause cytotoxicity.
Pharmacology, Toxicology and Pharmaceutical Science
7
13%
Agricultural and Biological Sciences
5
10%
Nursing and Health Professions
3
6%
Immunology and Microbiology
2
4%
Other
4
8%
Unknown
23
44%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 March 2018.
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#18,590,133
of 23,026,672 outputs
Outputs from Frontiers in Microbiology
#19,576
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#257,276
of 331,226 outputs
Outputs of similar age from Frontiers in Microbiology
#478
of 588 outputs
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