Title |
The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, March 2018
|
DOI | 10.3389/fcimb.2018.00072 |
Pubmed ID | |
Authors |
Huan Li, Feng Liu, Wei Peng, Kang Yan, Haixu Zhao, Ting Liu, Hui Cheng, Peixi Chang, Fangyan Yuan, Huanchun Chen, Weicheng Bei |
Abstract |
Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and the regulator CpxR, is known to be involved in the biofilm formation and virulence of Escherichia coli. However, the role of CpxA/CpxR remained unclear in Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show that CpxA/CpxR contributes to the biofilm formation ability of A. pleuropneumoniae. Furthermore, we demonstrate that CpxA/CpxR plays an important role in the expression of several biofilm-related genes in A. pleuropneumoniae, such as rpoE and pgaC. Furthermore, The results of electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analysis demonstrate that CpxR-P can regulate the expression of the pgaABCD operon through rpoE. In an experimental infection of mice, the animals infected with a cpxA/cpxR mutant exhibited delayed mortality and lower bacterial loads in the lung than those infected with the wildtype bacteria. In conclusion, these results indicate that the CpxA/CpxR TCS plays a contributing role in the biofilm formation and virulence of A. pleuropneumoniae. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 41 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 12% |
Student > Bachelor | 4 | 10% |
Researcher | 4 | 10% |
Student > Master | 4 | 10% |
Student > Doctoral Student | 2 | 5% |
Other | 5 | 12% |
Unknown | 17 | 41% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 9 | 22% |
Biochemistry, Genetics and Molecular Biology | 6 | 15% |
Agricultural and Biological Sciences | 4 | 10% |
Veterinary Science and Veterinary Medicine | 2 | 5% |
Nursing and Health Professions | 1 | 2% |
Other | 1 | 2% |
Unknown | 18 | 44% |