Carmelo Puglia, Paolo Blasi, Carmine Ostacolo, Eduardo Sommella, Claudio Bucolo, Chiara B. M. Platania, Giovanni L. Romano, Federica Geraci, Filippo Drago, Debora Santonocito, Barbara Albertini, Pietro Campiglia, Giovanni Puglisi, Rosario Pignatello
Abstract
Nanostructured lipid carriers (NLCs) loaded with palmitoylethanolamide (PEA) were formulated with the aim to enhance ocular bioavailability of PEA, particularly to the back of the eye. Technological characterization (e.g., size, charge) of NLC loaded with PEA formulation (PEA-NLC) was performed, and NLC morphology was characterized by electron microscopy. Ocular pharmacokinetic study, after topical administration of the formulation, was carried out in rabbit eye. Ultra-high performance liquid chromatography tandem mass spectrometry analysis was carried out to detect PEA levels in ocular tissues. Finally, the ocular tolerability of PEA-NLC formulation was assessed in rabbit eye. The novel formulation significantly increased PEA levels in ocular tissues compared to PEA suspension. Vitreous and retinal levels of PEA were significantly higher in the group treated with PEA-NLC formulation versus PEA suspension (PEA-NLC Cmax 5919 ± 541 pmol/g and 315 ± 70 pmol/g in vitreous and retina, respectively). The PEA-NLC formulation was characterized by high stability and robust ocular bioavailability. Therefore, this innovative formulation may be useful in clinical practice to manage retinal diseases.
Pharmacology, Toxicology and Pharmaceutical Science
18
31%
Chemistry
4
7%
Engineering
4
7%
Nursing and Health Professions
2
3%
Chemical Engineering
2
3%
Other
4
7%
Unknown
25
42%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2018.
All research outputs
#20,472,403
of 23,031,582 outputs
Outputs from Frontiers in Pharmacology
#10,248
of 16,347 outputs
Outputs of similar age
#291,304
of 329,889 outputs
Outputs of similar age from Frontiers in Pharmacology
#235
of 375 outputs
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