Chapter title |
The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins That Recognize Bent DNA.
|
---|---|
Chapter number | 12 |
Book title |
DNA-Protein Interactions
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2877-4_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2876-7, 978-1-4939-2877-4
|
Authors |
Stefanovsky, Victor Y, Moss, Tom, Victor Y. Stefanovsky, Tom Moss, Stefanovsky, Victor Y. |
Abstract |
So-called architectural DNA-binding proteins such as those of the HMGB-box family induce DNA bending and kinking. However, these proteins often display only a weak sequence preference, making the analysis of their DNA-binding characteristics difficult if not impossible in a standard electrophoretic mobility shift assay (EMSA). In contrast, such proteins often bind prebent DNAs with high affinity and specificity. A synthetic cruciform DNA structure will often provide an ideal binding site for such proteins, allowing their affinities for both bent and linear DNAs to be directly and simply determined by a modified form of EMSA. |
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