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Mendeley readers
| Chapter title |
Pulsed Field Gel Electrophoresis for Bifidobacterium
|
|---|---|
| Chapter number | 20 |
| Book title |
Pulse Field Gel Electrophoresis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2599-5_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2598-8, 978-1-4939-2599-5
|
| Authors |
Esther Jiménez, Marta Gómez, Laura Moles, Jiménez, Esther, Gómez, Marta, Moles, Laura |
| Abstract |
Pulse Field Gel Electrophoresis (PFGE), unlike conventional electrophoresis, can resolve DNA fragments greater than 30 kb and is a highly discriminatory molecular typing method. Here we describe a PFGE protocol for bifidobacteria characterized by a short lysis time determined by the addition of lysis reagents to the initial cell suspension, a reduced incubation period of the plugs in proteinase K, and an improved washing plug step with preheating of the buffer in a shaking incubator. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer | 1 | 33% |
| Unknown | 2 | 67% |
| Readers by discipline | Count | As % |
|---|---|---|
| Computer Science | 1 | 33% |
| Unknown | 2 | 67% |