@YasminDabiri @forum_gd Have you tried the pan-cytokine (Hu) stimulation kits? with blood gdTs, I've had success (see link below) expanding PBMCs using combination of bacterial antigen, IL-2+IL-15+IL-18 but not sure if this will help. Are you only looking
this is the correct link to Bruno's paper that blew my mind https://t.co/GmhmVluuP6
Then, I read this beautiful paper by @BSilva_Santos lab showing connections between CD27 expression and Tγδ 1 vs. 17 dichotomy (https://t.co/g400e5zHm7) and I found two things: 1) same for WC1+ γδ T cells and 2) different in cows than mice. OK, we're learn
This all started when I was working on another paper (https://t.co/g400e5zHm7) where I had discovered that blood γδ T cell clones can express WC1 genes (1 of 13, in cattle) differentially and this program is driven by what antigens the SRCR domains of WC1